Anabolic resistance protein malabsorption
Conditions
Interventions
Continuous intravenous stable isotope amino acid tracer infusion will be
applied, in combination with oral ingestion of 20g intrinsically labelled milk
protein, with plasma, muscle and duodenal muco
Sponsors
Medisch Universitair Ziekenhuis Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Male sex - Aged 18-35 years or 67+ years - Body mass index (BMI) between 18.5 and 30 kg/m2
Exclusion criteria
Exclusion criteria: - History of cardiovascular, respiratory, gastrointestinal, urogenital, neurological, psychiatric, dermatologic, musculoskeletal, metabolic, endocrine, haematological, immunologic disorders, allergy, major surgery and/or laboratory assessments which might limit participation in or completion of the study protocol, interfere with the execution of the experiment, or potential influence the study outcomes (to be decided by the principal investigator and responsible physician) - Major abdominal surgery interfering with gastrointestinal function (upon judgement of the principal investigator and responsible physician) - Use of medication which limit participation in or completion of the study protocol, interferes with the execution of the experiment, or potential influences the study outcomes (to be decided by the principal investigator and responsible physician) - Use of supplementation (i.e. vitamin, pre- and probiotic supplementation) within 14 days prior to testing - Administration of investigational drugs or participation in any scientific intervention study in the 14 days prior to the study, which may interfere with this study (upon judgement of the principal investigator and responsible physician) - Specific diet (e.g. vegetarian, vegan, gluten free, no diary) within the study period - Planning to lose weight during the study period - Lactose intolerance - Excessive alcohol consumption (defined as > 14 alcoholic consumptions per week) - Smoking - Drug use - Donated blood two months prior to the test day - Recent (
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. To assess the postprandial (0-5h) incorporation of dietary protein derived amino acids in duodenal epithelium in healthy young and older males. 2. To investigate whether age affects the postprandial (0-5h) incorporation of dietary protein derived amino acids in duodenal epithelium. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. To assess the postprandial (0-5h) plasma availability of dietary protein derived amino acids in healthy young and older males. 2. To assess postprandial (0-5h) fractional duodenal mucosal and muscle protein synthetic rates in healthy young and older males. | — |
Countries
Netherlands
Outcome results
None listed