kidney transplantation, renal transplantation
Conditions
Interventions
None listed
Sponsors
Radboud Universitair Medisch Centrum
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: Patient that will undergo a kidney transplantation with a kidney from a living donor. Age between 18 and 65 years old. Written informed consent
Exclusion criteria
Exclusion criteria: Chronic infections Diabetes mellitus Use of immunosuppressive medication (e.g. prednisone) or anti-inflammatory drugs (e.g. NSAIDs) within 3 months prior to study entry Clinically significant infections within 1 month prior to study (defined as fever >38.5) Hospital admission or surgery with general anaesthesia within 3 months prior to study entry Liver disease (ALAT more than three times upper reference limit) or known liver disease Previous vaccination within 1 month prior to study entry Inability to personally provide written informed consent (e.g. for linguistic or mental reasons) Inability to undergo PET-CT scanning History of hematological malignant disease Documented bleeding diathesis or thrombocytopenia <50 *10e9/L Pregnancy
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. The trained immunity profile of hematopoietic stem and progenitor cells from the bone marrow of the kidney transplant recipient, and the difference in this profile pre- and post- kidney transplantation. Before and 3 months after kidney transplantation, blood and bone marrow are collected from the patient and immune cells are isolated to examine the activity, transcriptome, and epigenome of hematopoietic stem and progenitor cells. 2. The metabolic activity of bone marrow from kidney transplant patients before and 3 months after kidney transplantation. FDG PET-CT imaging can be used to examine the metabolic activity of the bone marrow. 3. The trained immunity profile of monocytes from kidney transplant patients. Before and three months after kidney transplantation, blood is drawn from patients and circulating monocytes are isolated to examine the activity, transcriptome, and epigenome of these monocytes, and correlate with parameters measured in the bone marrow. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. The association between trained immunity and inflammation and tissue damage. The association between the trained immunity profile as measured in (1) and markers of tissue damage and systemic inflammation is examined. 2. The association between trained immunity and the concentration of immunosuppressive drugs in the blood of patients. 3. The association between trained immunity and the amount of white blood cells and subsets of white blood cells. | — |
Countries
Netherlands
Contacts
Public ContactR Duivenvoorden
Radboud Universitair Medisch Centrum
Outcome results
None listed