Costimulatory and co-inhibitory molecules on tumor-infiltrating lymphocytes from hepatocellular carcinoma and cholangiocarcinoma

Costimulatory and co-inhibitory molecules on tumor-infiltrating lymphocytes from hepatocellular carcinoma and cholangiocarcinoma - Costimulatory and co-inhibitory molecules in HCC and CCA

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON53120

Enrollment

272

Registered

2017-07-27

Start date

2018-01-03

Completion date

Unknown

Last updated

2024-04-23

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

hepatocellular carcinoma liver cancer

Interventions

None listed

Eligibility

Age

18 Years to 64 Years

Inclusion criteria

Inclusion criteria: Adult hepatocellular carcinoma (HCC) or cholangiocarcinoma (CCA) patients that will undergo surgery for this disease

Exclusion criteria

Exclusion criteria: Patients who refuse blood donation/participation in the study Patients with a severe immunocompromised condition, or patients taking immunosuppressive medication

Design outcomes

Primary

Measure	Time frame
The main study parameters are: 1) Frequencies and absolute numbers of different lymphocyte populations that together compose the tumor-infiltrating lymphocyte pool 2) Expression of costimulatory and co-inhibitory molecules on TIL versus lymphocytes isolated from tumor-free tissue versus circulating lymphocytes 3) Effect of blocking co-inhibitory molecules or stimulating costimulatory molecules expressed by TIL on their function (proliferation and cytokine production) in ex vivo culture experiments	—

Measure

Time frame

The main study parameters are: 1) Frequencies and absolute numbers of different lymphocyte populations that together compose the tumor-infiltrating lymphocyte pool 2) Expression of costimulatory and co-inhibitory molecules on TIL versus lymphocytes isolated from tumor-free tissue versus circulating lymphocytes 3) Effect of blocking co-inhibitory molecules or stimulating costimulatory molecules expressed by TIL on their function (proliferation and cytokine production) in ex vivo culture experiments

—

Secondary

Measure	Time frame
Depending on the co-inhibitory molecules detected on TIL, immunohistochemistry will be performed on residual formalin-fixed paraffin-embedded tumor tissue that is regularly stored to identify the expression of the ligands of these co-inhibitory molecules (e.g. PDL-1+2, GAL-9) on tumor (infiltrating) cells.	—

Countries

Netherlands

Outcome results