Pertussis Whooping cough
Conditions
Interventions
Volunteers will receive booster vaccination with Boostrix (GSK) after which
they will donate blood samples at various fixed time points.
Sponsors
Leids Universitair Medisch Centrum
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: Healthy competent adults, who have received recommended vaccinations according to the vaccination policy in the Netherlands
Exclusion criteria
Exclusion criteria: Subjects who meet any of the following criteria will be excluded from participation in this study: * has an auto-immune disease * has an immune deficiency * has a bleeding disorder * underwent splenectomy * receives immunosuppressive medication * receives medication which influences blood clotting * underwent active (clinically manifested) Bordetella pertussis infection at any time point in life or had contact with such a person * received Bp vaccination in the past 10 years * has Hb level below 8.5 mmol/L (male) or below 7.5 mmol/L (female) * is pregnant or breast feeding * had an allergic response to vaccination in the past * received any vaccination within the 14 days prior to the study * donated blood or plasma within the month prior to the study, or is planning to donate within the first month of the study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Detailed analysis of innate and adaptive immune cells and their kinetics in time will be performed by the means of flow cytometry. Flow cytometry-based approach will be developed to identify antigen specific memory B cells and plasma cells prior to analysis of their immunoglobulin receptors by the means of high throughput sequencing. Study will be finished while all donors are recruited. | — |
Countries
Netherlands
Outcome results
None listed