Digestie en absorptie Digestion & absorption Protein breakdown & availability
Conditions
Interventions
All subjects will perform two experiments in a double-blinded, randomized
order: ingest 40 g of milk protein with 5% glycation level in 600 mL water, or
40 g of milk protein with 50% glycation level
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: - Males - Aged between 18-35 years - Healthy, recreationally active (participating in recreational sports activities >= 1 and
Exclusion criteria
Exclusion criteria: - Smoking - Lactose intolerant or allergies to milk proteins - Musculoskeletal disorders - Metabolic disorders - Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications). - Chronic use of gastric acid suppressing medication or anti-coagulants - Unstable weight over the last three months - Diagnosed GI tract disorders or diseases - Blood donation in the past 2 months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary endpoint will be the appearance of milk protein-derived amino acids in plasma over the full assessment period (6 h), as determined using stable isotope tracer methodology. | — |
Secondary
| Measure | Time frame |
|---|---|
| To compare the impact of low versus high protein glycation on whole-body protein kinetics (total rate of appearance, exogenous rate of appearance, endogenous rate of appearance, rate of disappearance), whole-body protein metabolism (synthesis, breakdown, oxidation, net balance), peak plasma lysine concentration, overall plasma amino acid responses, plasma insulin-, and glucose responses. | — |
Countries
Netherlands
Outcome results
None listed