The effect of different radiotherapy regimen on the immune cell landscape

The effect of different radiotherapy regimen on the immune cell landscape - The effect of radiotherapy on immune cell landscape

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON52433

Enrollment

200

Registered

2019-04-11

Start date

2019-11-18

Completion date

Unknown

Last updated

2025-12-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

cancer tumor

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: - treated with radiotherapy for cancer at the Radboudumc - understand dutch - mentally competent - 30-80 years old

Exclusion criteria

Exclusion criteria: - autoimmune disease - immune- suppressive drugs (with the exception of

Design outcomes

Primary

Measure	Time frame
Immune cells will be characterized by flow cytometry, including CD4, CD8 and regulatory T cells, Natural Killer cells, MDSCs and macrophages. We will also look at the activation markers such as CD28, CD137 and CD66b, and the inhibitory markers PD1, PDL-1. RNA will be isolated from the immune cells for any specific gene expression analyzes or RNAseq. The effect of the various radiotherapy regimens on the dynamics of these immune cells will be determined.	—

Measure

Time frame

Immune cells will be characterized by flow cytometry, including CD4, CD8 and regulatory T cells, Natural Killer cells, MDSCs and macrophages. We will also look at the activation markers such as CD28, CD137 and CD66b, and the inhibitory markers PD1, PDL-1. RNA will be isolated from the immune cells for any specific gene expression analyzes or RNAseq. The effect of the various radiotherapy regimens on the dynamics of these immune cells will be determined.

—

Secondary

Measure	Time frame
Above mentioned immune cell markers will be quantified in biopsies using immunohistochemistry. The microbiome will be examined in faeces.	—

Countries

Netherlands

Outcome results