energy metabolism variance Mitochondrial dysfunction
Conditions
Interventions
None listed
Sponsors
Ziekenhuisvoorzieningen Gelderse Vallei
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: 1. Patient partipates in PRECISe trial 2. Adult >= 18 years-old admitted to the ICU 3. Unplanned ICU admission 4. Invasive mechanical ventilation initiated = 3 days
Exclusion criteria
Exclusion criteria: 1. Contraindication for enteral nutrition 2. Moribund or expected withholding of treatment 3. Kidney failure with *no dialysis*-code on admission 4. Hepatic encephalopathy 5. Body-mass index
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Autophagy flux measured in primary human myotubes incubated for 24 hours with serum from ICU patients, mitochondrial function in peripheral blood mononuclear cells (PBMCs) measured by functional respirometry (oroboros) and prostaglandin E2 (PGE2) levels as measured by LCMSMS. | — |
Secondary
| Measure | Time frame |
|---|---|
| - Investigate the localization of autophagy flux and of autophagy changes at different time points - To determine if and how parameters of the hypermetabolic inflammatory state are related to the progression of mitochondrial function in IC patients - To determine if and how progression of mitochondrial function is related to physical performance and clinical outcomes in IC patients - To examine how mitochondrial dynamics change over time and how this is associated with mitochondrial function in IC patients - Investigate the oxylipin profile measured by LCMSMS and lipopolysaccharide (LPS) levels - To examine how prostaglandin levels are related to inflammatory cytokine levels. - To determine how oxylipin levels are associated with changes in autophagy and mitochondrial function and vise versa. | — |
Countries
Netherlands
Outcome results
None listed