seroconversion arboviruses Viruses transmitted by mosquitoes
Conditions
Interventions
None listed
Sponsors
Universitair Medisch Centrum Utrecht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: In order to be eligible to participate in this study, a subject must meet all of the following criteria: Participating in the OH travel part I (OH travel part I is a non-wmo study 21-320) Providing a dried blood spot (DBS) that tested positive by serology. Positive samples are defined as samples that show reactivity against arbovirus NS1 or E proteins using a protein microarray.
Exclusion criteria
Exclusion criteria: Not willing to participate on part II
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Neutralising antibody titers against each of the targeted arboviruses at two different time- points since exposure and fold difference in antibody titers between time point 1 and 2. | — |
Secondary
| Measure | Time frame |
|---|---|
| From confirmed seropositive participants longitudinal B-cell profiles and functional antibody data will be generated. These data included enumerating the number of reactive clones, their cross-reactivity potential towards heterologous arboviruses and phenotypic and functional characterization of arbovirus-reactive clones. For these assays recombinant antibodies will be generated. These clones will be shared with the scientific community via the European virus archive - global, for assay standardization and quality control. Determinants of functional antibody response, such as age, gender and time since exposure might be also explored. | — |
Countries
Netherlands
Outcome results
None listed