Muscle and connective tissue research (no disorders) Connective tissue Muscle health
Conditions
Interventions
Participants will perform unilateral resistance exercise followed by the
ingestion of either 30 g of a protein blend or a non-caloric placebo (flavored
water). Continuous intravenous tracer infusion
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: - Males - Aged between 18-35 years - Healthy, recreationally active (performing exercise between 1 and 4 times per week in the past 6 months) - BMI 18.5 - 30 kg/m2 - No physical limitations (i.e. able to perform all activities associated with daily living in an independent manner).
Exclusion criteria
Exclusion criteria: - Females - Musculoskeletal disorders - Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications). - Participation in any structured regular exercise program - Chronic use of gastric acid suppressing medication or anti-coagulants - Pathologies of the gastrointestinal tract - Blood donation in the past 2 months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| - Myofibrillar protein-bound L-[ring-13C6] phenylalanine enrichment (expressed as MPE) - Muscle connective tissue protein-bound L-[ring-13C6] phenylalanine enrichment (expressed as MPE) - Average plasma L-[ring-13C6] phenylalanine enrichment over the 3.5 h period before the study beverage and 5 h postprandial period. | — |
Secondary
| Measure | Time frame |
|---|---|
| * Plasma amino acid concentrations (expressed as *mol/L) * Total plasma amino acids (AAmax [*mol/L]) * Plasma glucose (glucosemax [mmol/L]) * Plasma insulin (insulinmax [mU/L]) | — |
Countries
Netherlands
Outcome results
None listed