veroudering ageing senescence
Conditions
Interventions
Twenty grams of three deuterium (2H) - labelled protein sources (milk, sorghum
and black beans) will be consumed on three different test days. The meals will
be divided into 10 portions, consumed ev
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Age: 20-35 years or 65-80 years - Body Mass Index (BMI): 20.0 - 30.0 kg/m2 - Veins suitable for blood sampling - Healthy as assessed with a questionnaire - Regular and normal Dutch eating habits as assessed with a questionnaire - Having given written informed consent - Willing to comply with study procedures - Accept use of all encoded data, including publication, and the confidential use and storage of all data for 15 years.
Exclusion criteria
Exclusion criteria: - Chronic disease, for example: o Diabetes mellitus, being treated for high blood glucose or increased fasting blood glucose (> 6.7 mmol/l in finger prick blood) o Active cardiovascular disease o Hepatic disease (e.g. hepatitis) o Renal disease o Cancer o Bowel disease (e.g. inflammatory bowel disease, ulcers, bleeding) o Pancreatitis - History of medical or surgical events that may affect GI function and the study outcomes, for example: o Bariatric surgery o Gastrointestinal tract surgery o Digestive tract disorder o Chewing problems - Medicine use that interferes with GI function and the study outcomes, for example: o Glucose lowering drugs o Proton pump inhibitors o Laxatives - Habits that interfere with the study outcomes: o Probiotics and/or protein supplement use o Smoking o Drug abuse o Alcohol consumption for men >21 units/week and >4/day and for women >14 units/week and >3/day o Following a weight-loss diet, medically prescribed diet or other diet with a low calorie intake or an unbalanced nutrient intake like a vegan or very low carbohydrate diet o Moderate to high intense physical activity for more than 5 hours a week o Difficulties with eating breakfast in the morning - Other: o Self-reported allergy or intolerance to the tested products (milk, sorghum, black beans) o Weight loss of more than 3 kg in the month prior to study screening o Being pregnant or breastfeeding o For men: Hb
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Blood samples will be drawn every hour until the 5th hour and every 30 minutes afterwards up to 8 hours. Plasma and meal amino acids enrichment will be measured with gas chromatography - isotope ratio mass spectrometry (GC-IRMS) for both isotopes. The ratio between 2H and 13C labelled amino acids in both meal and blood plasma will be determined and amino acid digestibility of the tested protein source will be calculated. | — |
Secondary
| Measure | Time frame |
|---|---|
| - To determine the difference in amino acid digestibility between milk, sorghum and black beans within age groups. - To explore the difference in blood amino acids kinetics between milk, sorghum and black beans and between older and younger adults. - To investigate the association between gut health as measured with blood markers and amino acid digestibility of milk, sorghum and black beans. | — |
Countries
Netherlands
Outcome results
None listed