gezonde proefpersonen n.a.
Conditions
Interventions
None listed
Sponsors
Leids Universitair Medisch Centrum
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: - *18 years old - BMI *18,5 and *25kg/m2
Exclusion criteria
Exclusion criteria: - Chronic disease or active infection - Medication use other than contraceptive, during the last 12 weeks prior to inclusion. - A history of allergy - Blood or plasma donation in the last 12 weeks prior to inclusion. - Participation in other medical research in the last 12 weeks prior to inclusion.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The changes in gene expression profile and pathway signaling in white blood cells using RNA nanostring analysis on whole blood samples. The blood samples are collected at 3 different time points in a fed state, fasted state and after the second cycle of the fasting mimicking diet. | — |
Secondary
| Measure | Time frame |
|---|---|
| - Flow cytometry on peripheral blood mononuclear cells, including confirmation of gene expression profile alterations on the protein level. - Change in plasma concentration of IGF1, glucose and ketone bodies. - Evaluation of experienced side effects from FMD recorded in the toxicity forms in the Case report form (CRF) assessed on day 1 and 31. | — |
Countries
Netherlands
Outcome results
None listed