COVID-19 SARS-CoV-2
Conditions
Interventions
Patients who have given informed consent for participation in this study will
undergo surgery. The bariatric surgery procedure will be performed normally, no
modifications are necessary. After intro
Sponsors
Universitair Medisch Centrum Groningen
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: Intervention group (Obese) Healthy participants who give informed consent to participate in this study that are male and female, between aged between 18 and 65 years old with a BMI above 40. Control group (Lean) The control group will consist of participants that give informed consent to participate in this study, male and female aged between 18 and 65 years old, without comorbidities, no cancer and a BMI of 25 or less.
Exclusion criteria
Exclusion criteria: For both groups: Diabetes mellitus, hypertension, metabolic syndrome, asthma, immune disease such as Crohn colitis, rheumatic disease, cancer, use of soft/hard drugs or smoking. Previous COVID-19 infection.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Main study parameters/endpoints : 1. To determine if adipose tissue is permissive to infection by plaque assay and qPCR of viral RNA. 2. To determine the mRNA and protein expression of SARS-CoV-2 receptors such as ACE2 and to perform blocking experiments to elucidate the entry receptor. 3. To determine if there is an exaggerated inflammatory response upon SARS-CoV-2 infection by examining the mRNA levels of inflammatory mediators and adipokines. As well as the protein levels of adipose-tissue derived factors and inflammatory mediators released upon SARS-CoV-2 infection in culture medium by Luminex analysis. | — |
Countries
Netherlands
Outcome results
None listed