Eiwitbehoefte Protein requirement
Conditions
Interventions
All subjects will consume a test diet containing 0.1, 0.3, 0.6, 0.9, 1.2, 1.5,
and 1.8 g protein/kg/d during acute metabolic trials.
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: - Male - Aged between 18-35 years - Healthy, recreationally active (exercise at least 1 per two weeks and maximum 4 days a week) - 18.5
Exclusion criteria
Exclusion criteria: - Smoking - Musculoskeletal disorders - Metabolic disorders - Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications). - Chronic use of gastric acid suppressing medication or anti-coagulants - Unstable weight over the last three months - Diagnosed GI tract disorders or diseases - Blood donation in the past 2 months
Design outcomes
Secondary
| Measure | Time frame |
|---|---|
| To determine carbon dioxide production, 13CO2 production, plasma and urine L-[1-13C]-phenylalanine enrichments, and plasma and urine phenylalanine concentration in a young, healthy, male population when applying the IAAO technique. | — |
Primary
| Measure | Time frame |
|---|---|
| The primary endpoint is the estimated average requirement (EAR) and recommended daily allowance (RDA) for dietary protein, which will be determined using the IAAO method by measuring 13C enrichment in expired breath and enrichment of L-[1-13C]-phenylalanine in plasma and urine. | — |
Countries
Netherlands
Outcome results
None listed