vitamine A deficientie Vitamin A deficiency
Conditions
Interventions
Subjects will be randomly allocated to four treatment groups of equal size
(n=4
group A*D). All subjects will be provided with a standardised meal on the
evening before start of the study which they will consume at home between 18h00
and 20h00. They will additionally be asked t
group B, 300 µg
group C, 500 µg
and group D, 800
µg. Additional fasting blood samples will be collected at day 7, day 14, day 21
and day 28 (end point). Subjects will be asked not to deviate from their
habitual diets during the s
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Female Dutch spoken Age: 18-35 years BMI: 18.5-25 kg/m2 Non-smoking
Exclusion criteria
Exclusion criteria: Pregnant or lactating Metabolic disease (e.g. Diabetes Mellitus, hypercholesterolemia) Disorder or use of drugs that interferes with fat-soluble vitamin absorption History of chronic diseases (e.g. cancer, renal disease, liver disease, gastrointestinal disorders) Food allergies related to food that will be provided during the study Recent (past 6 months) surgery, blood transfusion and blood donation Dieting or having irregular dietary habits Use of (multi)vitamin preparations with vitamin A or beta-carotene (
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Percentage of 13C10-labelled retinol in plasma with intakes of varying doses of unlabelled retinol over time (4 weeks), and the shape of the dose response curve. In addition, we will measure the area under the curve (AUC) of 13C10-labelled *-carotene and 13C5-labelled retinol in blood plasma over a 24h period. | — |
Secondary
| Measure | Time frame |
|---|---|
| - Serum retinol concentration (µmol / L) - *-carotene bioconversion (%) - Inflammatory markers (C-reactive protein (CRP), alpha-glycoprotein (AGP) - Dietary energy and vitamin A intake - Genetic variants in the bco1 gene | — |
Countries
Netherlands
Outcome results
None listed