respiratoir syncytieel virus bronchiolitis RSV-bronchiolitis
Conditions
Interventions
None listed
Sponsors
Academisch Medisch Centrum
Eligibility
Age
2 Years to 11 Years
Inclusion criteria
Inclusion criteria: - Age between 2 and 24 months - No known pulmonary disease - Endotracheal intubation (for elective surgery) - No signs of infection (fever, temperature >38.5 C) and no respiratory symptoms (cough, wheeze, snotty) present at moment of intubation. - No reported respiratory symptoms in the last 7 days prior to surgery (cough, wheeze, snotty) - Informed consent obtained for both non-endoscopic cytobrushing and serological testing after confirmation of comprehension of the Dutch language.
Exclusion criteria
Exclusion criteria: - Age 24 months - Reported pulmonary disorder - Non-elective endotracheally intubation - Symptoms of a present or recent infection (T>38.5C in the last 7 days prior to surgery) or reported respiratory symptoms in the last 7 days prior to surgery - No consent.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| We will use the collected cells to obtain pediatric HAE cultures. We will use these cultures as an ex vivo model to study NETs induced changes in the context of RSV infection of the pediatric airways. We will perform measurements on airway mucus (protein expression) and airway epithelial damage (necrosis, apoptosis, loss of differentiation, loss of barrier function, apoptotic gene expression, inflammation markers and inflammatory pathways). | — |
Secondary
| Measure | Time frame |
|---|---|
| n.a. | — |
Countries
Netherlands
Outcome results
None listed