Hepatic glycogen Storage of sugar in the liver
Conditions
Interventions
Short overnight fasting protocol
Subjects will receive a standardized snack at 16.30. At 23.00 subjects will
receive their standardized diner, where after subjects stay fasted for 9.5
hours.
Long o
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Participants are able to provide signed and dated written informed consent prior to any study specific procedures - Caucasian (people will be excluded when having a *50% racial African/Asian background) - Participants should have suitable veins for cannulation or repeated venipuncture - Women are post-menopausal (defined as at least 1 year post cessation of menses) - Men and women aged * 45 and * 75 years at the start of the study - Body mass index (BMI) 18.5 * 25 kg/m2 - Stable dietary habits (no weight loss or gain >3kg in the past 3 months) - Sedentary lifestyle (not more than 3 hours of vigorous sports per week) - Liver fat content
Exclusion criteria
Exclusion criteria: - Type 2 Diabetes Mellitus - Active diseases (cardiovascular, diabetes, liver, kidney, cancer or other) - Any contraindication for MRI scanning - Alcohol consumption of >2 servings per day - Regular smoking - No use of medication interfering with investigated study parameters (as determined by responsible physician) - Participants who do not want to be informed about unexpected medical findings, or do not wish that their physician
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main study outcome is the change in hepatic glycogen in response to a 9.5hrs and a 16 hrs fast, measured with 13C-magnetic resonance spectroscopy (MRS). | — |
Secondary
| Measure | Time frame |
|---|---|
| Whole body fat oxidation measured using the respiratory quotient (VCO2/VO2) during the night. Oxygen utilization and carbondioxide production are measured using the respiration chamber. | — |
Countries
Netherlands
Outcome results
None listed