Immune responses in a rural to urban gradient: identifying geographical footprints of the immune system to improve vaccine development

Immune responses in a rural to urban gradient: identifying geographical footprints of the immune system to improve vaccine development - Geographical differences in immune responses

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON48968

Enrollment

Registered

2019-06-28

Start date

2019-07-25

Completion date

Unknown

Last updated

2024-04-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Immunological profile

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: In order to be eligible to participate in this study, a subject must meet all of the following criteria: - Living in the urban, semi-urban or rural setting preferably lifelong (not less than 10 years) - Be 18-40 years of age

Exclusion criteria

Exclusion criteria: A potential subject who meets any of the following criteria will be excluded from participation in this study:;- Any history of diabetes, arterial hypertension, chronic renal failure - Signs or history of infection at the time of inclusion - Any history or clinical signs of inflammatory diseases

Design outcomes

Primary

Measure	Time frame
Primary endpoint * The percentages of interleukin (IL-) 4, IL-5, IL13 cytokine producing CD4+ T cells as indicator of Th2 response and interferon gamma (IFNg) producing CD4+ T cells as Th1 cells, in PBMC from Europeans with those of Africans living in urban, semi urban and rural areas	—

Secondary

Measure	Time frame
Secondary endpoint * Characterization of PBMCs in terms of lineages of T cells, B cells, monocytes and dendritic cells as well as innate lymphocytes and their subsets and clusters in Europeans in addition to Africans living in urban, semi urban and rural areas. Exploratory endpoints * Data set on transcriptome of cells identified to pay a determining role in European, African rural, semi urban and rural profiles. * Combined analysis of the high dimensional data obtained by mass cytometry and RNA sequencing * Establishment of SOPs to be used at the Cheikh Anta Diop University in Senegal for further investigation of vaccine responses	—

Measure

Time frame

Secondary endpoint * Characterization of PBMCs in terms of lineages of T cells, B cells, monocytes and dendritic cells as well as innate lymphocytes and their subsets and clusters in Europeans in addition to Africans living in urban, semi urban and rural areas. Exploratory endpoints * Data set on transcriptome of cells identified to pay a determining role in European, African rural, semi urban and rural profiles. * Combined analysis of the high dimensional data obtained by mass cytometry and RNA sequencing * Establishment of SOPs to be used at the Cheikh Anta Diop University in Senegal for further investigation of vaccine responses

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Countries

Netherlands

Outcome results