CyTOF and microbiota analysis on Crohn*s disease-associated and idiopathic perianal fistulas

CyTOF and microbiota analysis on Crohn*s disease-associated and idiopathic perianal fistulas - CyTOF and microbiome analysis fistulas

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON48904

Enrollment

Registered

2018-09-18

Start date

2019-01-08

Completion date

Unknown

Last updated

2024-04-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

fistulas Perianal fistulas

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: In order to be eligible to participate in the *Crohn-fistula group* of this study, a subject must meet all of the following criteria: - Patients with perianal fistulas who will have surgery for their perianal fistula including scraping of the fistula. - Patients with confirmed Crohn*s disease (clinical and endoscopic). In order to be eligible to participate in the *non-Crohn-fistula group* of this study, a subject must meet all of the following criteria: - Patients with perianal fistulas who will have surgery for their perianal fistula including scraping of the fistula. - Patients with no clinical suspicion for IBD (based on the fecal calprotectin (FCP)) or no signs of inflammation on endoscopy.

Exclusion criteria

Exclusion criteria: A potential subject who meets any of the following criteria will be excluded from participation in this study: - In the *non-CD-fistula group* perianal fistulas cannot be the result of pelvic malignancy or radiation therapy or another known medical condition in the pelvic region.

Design outcomes

Primary

Measure	Time frame
Differences in immune cell populations analysis between fistula tracts from patients with and without Crohn*s disease.	—

Secondary

Measure	Time frame
Differences in immune cell population in rectum biopsies from patients with and without Crohns disease. Correlation of immune cell populations in the fistula tracts with immune cells in peripheral blood and rectum biopsies. Differences in microbiota between fistula tracts from patients with and without Crohns disease. Differences in the microbiota of the gut and the fistula tract. Differences in fibroblast expression patterns between fistula tracts from patients with and without Crohn's disease Differences in the fibroblast expression patterns of fistula tract and the rectum biopsies	—

Measure

Time frame

Differences in immune cell population in rectum biopsies from patients with and without Crohn*s disease. Correlation of immune cell populations in the fistula tracts with immune cells in peripheral blood and rectum biopsies. Differences in microbiota between fistula tracts from patients with and without Crohn*s disease. Differences in the microbiota of the gut and the fistula tract. Differences in fibroblast expression patterns between fistula tracts from patients with and without Crohn's disease Differences in the fibroblast expression patterns of fistula tract and the rectum biopsies

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Countries

Netherlands

Outcome results