Center for Translational Pediatric Pulmonology Modelling the bronchial epithelium in children

Center for Translational Pediatric Pulmonology *Modelling the bronchial epithelium in children* - CTPP

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON48628

Enrollment

Registered

2019-05-21

Start date

2020-11-23

Completion date

Unknown

Last updated

2025-10-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

asthma, cystic fibrosis, preschool wheeze

Interventions

None listed

Eligibility

Age

No minimum to 17 Years

Inclusion criteria

Inclusion criteria: children requiring (laryngo-tracheo-)brochoscopy for clinical indications scopy will take place during office hours

Exclusion criteria

Exclusion criteria: children with malignancies requiring bronchoscopychildren who have had a (lung) transplantation in the past.Children younger than 1 year old: the diameter of their airway does not allow introduction of a bronchoscope with sufficient size working channel for the biopsy forceps and the higher risk of anaesthesia in this groupChildren and parents who cannot read the Dutch patient information.

Design outcomes

Primary

Measure	Time frame
• To establish a model of the bronchial epithelium by epithelial organoid cultures derived from the bronchial and nasal brush, as well as bronchial and nasal brushes of the pediatric airways. We will validate these models using comparative RNA seq with matched single cell RNA seq of airway wall biopsies.	—

Secondary

Measure	Time frame
• Comparative genomics of nasal and bronchial epithelial cells in children. Using gene expression and epigenetic DNA markers of epithelial cells of the upper and lower airways, we will describe which markers of the upper airway (nose) can be used as a valid reflection of the lower airways by validating them against matched single cell RNA seq of airway wall biopsies. We will publish a catalogue of gene expression signatures in children that reflect the bronchial epithelium that can be used by researchers all across the world. • Establishment of an Epithelial Organoid Repository for mechanistic and interventional pediatric respiratory research. • To describe the single cell composition of the airway wall in pediatric respiratory disease, including asthma, severe wheeze, and cystic fibrosis (CF). As airway wall biopsies contain 50 - 70 % epithelial cells, this may serve as an excellent validation of our epithelial organoids and provide more insight into the cell types and states of pediatric respiratory disease.	—

Measure

Time frame

• Comparative genomics of nasal and bronchial epithelial cells in children. Using gene expression and epigenetic DNA markers of epithelial cells of the upper and lower airways, we will describe which markers of the upper airway (nose) can be used as a valid reflection of the lower airways by validating them against matched single cell RNA seq of airway wall biopsies. We will publish a catalogue of gene expression signatures in children that reflect the bronchial epithelium that can be used by researchers all across the world. • Establishment of an Epithelial Organoid Repository for mechanistic and interventional pediatric respiratory research. • To describe the single cell composition of the airway wall in pediatric respiratory disease, including asthma, severe wheeze, and cystic fibrosis (CF). As airway wall biopsies contain 50 - 70 % epithelial cells, this may serve as an excellent validation of our epithelial organoids and provide more insight into the cell types and states of pediatric respiratory disease.

—

Countries

Netherlands

Contacts

Public ContactI.F.A. Bocca-Tjeertes

Universitair Medisch Centrum Groningen

i.tjeertes@umcg.nl0503616161

Outcome results