Searching for non-invasive Magnetic Resonance-based markers for CrAT activity and carnitine availability

Searching for non-invasive Magnetic Resonance-based markers for CrAT activity and carnitine availability - Markers of CrAT protein activity and carnitine availability

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON48547

Enrollment

Registered

2018-10-10

Start date

2019-03-21

Completion date

Unknown

Last updated

2024-04-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Phosphocreatine kinetic and exercise metabolism

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: - Males - Generally healthy (not cardiovascular complications) - Age 18-40 years old - BMI 18 - 28 kg/m2 - No medication use that interfere with the aims of the study - Stable level of daily physical activity or training for at least 6 months

Exclusion criteria

Exclusion criteria: - Regular smokers, drug abuse - Participation in other studies - Weight gain/loss > 3 kg in the last 6 months - Contraindications for MRS scans - Vegetarian eating behavior - Females

Design outcomes

Primary

Measure	Time frame
- Skeletal muscle phosphocreatine on-kinetic (pmol / kg tissue) at the beggining of exercise measured by MRS technique - Protein content and activity of carnitine acetyltransferase (CrAT) determined in muscle biopsy	—

Secondary

Measure	Time frame
- Acetylcarnitine levels in skeletal muscle measured by MRS technique (mmol/kg wet weight) at resting and post exercise condition - Muscle tissue oxygenation measured by Blood Oxygen level-dependent (BOLD) MRI technique. - Ex vivo skeletal muscle mitochondrial function expressed as O2 consumption in pmol/mg/min, determined from muscle biopsy sample - Total carnitine availability and protein content, expression/activity of regulatory enzyme involved in the skeletal muscle acetylcarnitine formation (determined in muscle biopsies)	—

Measure

Time frame

- Acetylcarnitine levels in skeletal muscle measured by MRS technique (mmol/kg wet weight) at resting and post exercise condition - Muscle tissue oxygenation measured by Blood Oxygen level-dependent (BOLD) MRI technique. - Ex vivo skeletal muscle mitochondrial function expressed as O2 consumption in pmol/mg/min, determined from muscle biopsy sample - Total carnitine availability and protein content, expression/activity of regulatory enzyme involved in the skeletal muscle acetylcarnitine formation (determined in muscle biopsies)

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Countries

Netherlands

Outcome results