metabolisme immune response postprandial effects to differences in fat structure
Conditions
Interventions
The dietary lipid challenge tests will be provided in the form of a liquid
shake (0.6 L). Each shake will contain 95 gram of fat. The three types of fat
that will be tested include: a) 100% vegetabl
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Apparently healthy man or woman - Age 40-70y at the time of recruitment - BMI of 22-27 kg/m2 - Having a Hb value above 8.4 (men) or 7.4 (women) mmol/L (will be checked at the screening visit) - Having veins suitable for blood sampling via a catheter cannula (judged by study nurse/ medical doctor) - Having a general practitioner - Signed informed consent
Exclusion criteria
Exclusion criteria: - Any chronic metabolic, gastrointestinal, inflammatory or chronic disease (such as diabetes, anaemia, hepatitis, cardiovascular disease) - History of gastro-intestinal surgery or having (serious) gastro-intestinal complaints - Renal or hepatic malfunctioning (pre-diagnosis or determined based on ALAT, ASAT and creatinine values) - Use of medication that may influence the study results, such as laxatives, stomach protectors and drugs that can affect intestinal motility. - Donated or intend to donate blood from 2 months before the study until the end of the study - Reported slimming, medically prescribed or vegan diet - Unstable body weight (weight gain or loss >5 kg in the past three months) - Current smokers - Alcohol on average: more than 2 consumptions/day or more than 14 consumptions/week - Pregnant, lactating or wishing to become pregnant in the period of the study (self-reported) - Use of drugs (soft and/or hard drugs) - Food allergies for products that we use in the study - Participation in another clinical trial at the same time, or in the month preceding the start of this study - Inability to understand study information and/or communicate with staff - Members of the research team - Working, or doing an internship or thesis at the division *Human Nutrition and Health*, Wageningen University
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1) whole genome gene expression changes in isolated monocytes from the circulation, 2) postprandial changes in cytokine concentrations in the circulation, and 3) functional responses and sensitivity of the isolated monocytes from the circulation. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1) postprandial triglyceride concentration and other cardio-metabolic markers in the circulation, 2) postprandial feelings of hunger and satiety, and 3) changes in cytokine concentrations in the circulation measured in dried blood spots. | — |
Countries
Netherlands
Outcome results
None listed