microbiota compositie activiteit en microbiële producten microbial activity products of gut bacteria
Conditions
Interventions
200 mL tap water, 5 gram fructo-oligosaccharides, 5 gram
galacto-oligosaccharides, non-absorbable marker PEG-4000
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: • Males • Age 18-60yrs • BMI between 18.5 and 30 kg/m2 • Regular bowel movement (defaecation on average once a day) • Signed informed consent
Exclusion criteria
Exclusion criteria: • Having a history of medical or surgical events that, in the opinion of the Investigator, may either put the subject at risk because of participation in the study, or influence the results of the study (e.g. diabetes, cardiovascular disease, gastrointestinal disease, renal failure, cancer, infectious disease, nose/throat). * Having a history of surgical events of the gastro-intestinal tract (e.g. bariatric surgery/gastric bypass surgery). • Presence of swallowing disorder • Use of any prescribed or non-prescribed medication (other than paracetamol) including antacids, analgesics, and herbal remedies during the three (3) weeks prior to study start. • Use of cholesterol lowering medication • Carrying a pacemaker or any other (implanted) medical electronic device • Smoker • Unstable body weight (weight gain or loss >5kg in the past 3 months prior to the study start) • Use of antibiotics within 3 months of starting the study or planned during the study • Use of pro- or prebiotics (e.g. galacto-oligosaccharides, fructo-oligosaccharides) • Constipation/infrequent bowel movement • Abuse of drugs/alcohol (alcohol: >4 consumptions/day or >21 consumptions/week) • Participation in another biomedical study • Having diarrhoea within 2 months prior to the study start • Personnel of Wageningen University, Division of Human Nutrition, their partner and their first and second degree relatives • Current participation in other research from the Division of Human Nutrition • Not willing to undergo fluoroscopy • Having blood vessels that are too difficult for inserting a venflon * Having a hemoglobin of
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Main study parameters/endpoints: (isotopic) enrichments of SCFAs (in the cecum and blood), and plasma organic acids, glucose, cholesterol, fatty acids | — |
Secondary
| Measure | Time frame |
|---|---|
| Blood markers for microbial activity: Markers of specific microbial group activity that can be related to the host health status, like organic acids, hippurate, di- en tri-methylamine, acetaldehydes, glucuronic acid conjugates, and bile acid (conjugates). Bacterial activity/microbiota composition in luminal samples. Fibre degradation: To study acute fibre breakdown, the fibre degradation products, including mono-, di-, tri-, oligo- and polysaccharides. Urine metabolic profiles: A fasting urine sample will be collected before at the start of the test day and at the end of the test day. Urine samples will be analysed to analyse bile acids, organic acids, aminoa cids, including bacterial products and their taurine, glycine, and glucuronic acid conjugates. | — |
Countries
Netherlands
Outcome results
None listed