Effects of IVF culture conditions on the embryonic genome, methylome and transcriptome: a study to evaluate the safety and quality of different culture systems

Effects of IVF culture conditions on the embryonic genome, methylome and transcriptome: a study to evaluate the safety and quality of different culture systems - IVF culture conditions and embryonic multi-omics

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

NL-OMON

Registry ID

NL-OMON48079

Enrollment

219

Registered

2020-01-16

Start date

2020-03-16

Completion date

Unknown

Last updated

2025-09-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

subfertiliteit subfertility

Interventions

The embryo's will be cultured in the medium and oxygen level they were cultured in before freezing. This can be one of the following media: HTF, G3, G5, Sydney IVF Medium, and one of the following o

Eligibility

Inclusion criteria

Inclusion criteria: - MUMC: embryos from couples who took part in the MEDIUMtrial-0 or the MEDIUMtrial-1 - UMCG: embryos from couples who underwent an IVF treatment between January 2012 and December 2013.

Exclusion criteria

Exclusion criteria: - embryo's that do not survive the thawing procedure

Design outcomes

Primary

Measure	Time frame
a) To evaluate the effect of 4 (previously) widely used IVF culture media (Sydney IVF Medium, G3, G5 and HTF) on the genome, epigenome (mainly methylome and chromatin accessibility) and transcriptome of human pre-implantation embryos. These culture media have been associated with differences in early life outcomes. b) To evaluate the effect of preimplantation human embryo culture at different oxygen concentrations (5% and 20% oxygen), on the genome, epigenome (mainly methylome and chromatin accessibility) and transcriptome of pre-implantation human embryos. Both concentrations have been widely used in human ART.	—

Measure

Time frame

a) To evaluate the effect of 4 (previously) widely used IVF culture media (Sydney IVF Medium, G3, G5 and HTF) on the genome, epigenome (mainly methylome and chromatin accessibility) and transcriptome of human pre-implantation embryos. These culture media have been associated with differences in early life outcomes. b) To evaluate the effect of preimplantation human embryo culture at different oxygen concentrations (5% and 20% oxygen), on the genome, epigenome (mainly methylome and chromatin accessibility) and transcriptome of pre-implantation human embryos. Both concentrations have been widely used in human ART.

—

Secondary

Measure	Time frame
c) To establish whether there are links between the epigenome (mainly methylome and chromatin accessibility), genome and transcriptome that could explain high levels of CIN seen in human IVF embryos. d) To develop in silico methods for lineage tracing that can be used to determine the fate of chromosomally abnormal cells during embryo development.	—

Countries

Netherlands

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)