atherosclerosis
Conditions
Interventions
A single high-fat challenge (milkshake containing 95g of fat) and *control*
shake (comparable to an average breakfast).
Sponsors
Radboud Universitair Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Age between 18 and 40 years LDL cholesterol
Exclusion criteria
Exclusion criteria: Smoking within the year before study entry Diagnosed with any long-term medical condition that can interfere with the study (i.e. renal failure, galbladder disease, cardiovascular disease, diabetes, rheumatoid arthritis etc.) Medication (with the exception of oral contraceptives) or supplement use (i.e. omega3) BMI 27 kg/m2 Previous vaccination within 3 months prior to study entry Current infection or clinically significant infections within 1 month before study entry (defined as fever > 38.5°C) Allergic to cow milk/dairy products Pregnancy/lactation Abuse of alcohol or drugs Vegetarian diet
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Blood will be drawn at t=0h (before) and at t=1h, t=2h, t=4h, t=6h, t=24 and t=72h after an oral fat load and at the same time points after a *control* shake. The primary endpoint is the monocyte TNFa production upon ex vivo stimulation with LPS (TLR4 ligand) at t=72h. | — |
Secondary
| Measure | Time frame |
|---|---|
| Additional secondary endpoints are the monocytes* inflammatory phenotype as assessed by flowcytometry analysis , epigenetic and metabolic reprogramming and serum induced inflammation. | — |
Countries
Netherlands
Outcome results
None listed