Systemic and local inflammation in burn wound patients.

Systemic and local inflammation in burn wound patients. - Systemic and local inflammation in burns. Acronym: SLIB

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON47865

Enrollment

120

Registered

2016-04-07

Start date

2016-05-04

Completion date

Unknown

Last updated

2025-08-18

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

partial and full-thickness burns

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Patient group >=18 years Competent and temporarily incompetent patients Informed Consent by patient or the legal representative No acute psychiatric disorders Speak sufficient Dutch' Healthy volunteers: 18 years or older competent informent consent sufficient dutch proficiency

Exclusion criteria

Exclusion criteria: patients: See inclusion criteria Healty volunteers: any medication exept contraceptives known underlying diseases as cancer, inmune deficiency, hypertension, complement disorder

Design outcomes

Primary

Measure	Time frame
Main objectives are to analyse components of the complement system and inflammatory cells involved in the different inflammatory mechanisms and systems described above. These include complement C3, C4, C5 and NETs levels (cell free DNA (cfDNANA)) in blood at different time points between day 0 and 30 pb and when the patient has to undergo escharectomy in eschar obtained at the day of surgical procedure. Timeframes for analyses are: day 0, 3-6, 7-14 and 21- 30. The latter time points will only apply for patients with more severe burn wounds and hospitalisation during the entire period. The analysis will be performed by ELISA. In addition complement (e.g. C3, C4, C5) as well as neutrophils (MPO), macrophages (CD68), lymphocytes (CD45), thrombi (CD31), NETosis (H3 histon citrulline), tissue factor (TF) and dipeptidyl peptidase 4 (DPP4/CD26) will be analysed in burned tissue samples (eschar collected at the day of the planned surgical procedure) by using (immuno) histochemistry for the cells and FACS analysis.	—

Measure

Time frame

Main objectives are to analyse components of the complement system and inflammatory cells involved in the different inflammatory mechanisms and systems described above. These include complement C3, C4, C5 and NETs levels (cell free DNA (cfDNANA)) in blood at different time points between day 0 and 30 pb and when the patient has to undergo escharectomy in eschar obtained at the day of surgical procedure. Timeframes for analyses are: day 0, 3-6, 7-14 and 21- 30. The latter time points will only apply for patients with more severe burn wounds and hospitalisation during the entire period. The analysis will be performed by ELISA. In addition complement (e.g. C3, C4, C5) as well as neutrophils (MPO), macrophages (CD68), lymphocytes (CD45), thrombi (CD31), NETosis (H3 histon citrulline), tissue factor (TF) and dipeptidyl peptidase 4 (DPP4/CD26) will be analysed in burned tissue samples (eschar collected at the day of the planned surgical procedure) by using (immuno) histochemistry for the cells and FACS analysis.

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Secondary

Measure	Time frame
-	—

Countries

Netherlands

Outcome results