Effect of storage time and additive solution on post-transfusion clearance and metabolic recovery

Effect of storage time and additive solution on post-transfusion clearance and metabolic recovery - Metabolic recovery of transfused RBCs

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

NL-OMON

Registry ID

NL-OMON47236

Enrollment

Registered

2017-01-31

Start date

2017-08-17

Completion date

Unknown

Last updated

2024-06-11

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Effectiviteit van rode bloedceltransfusie blood transfusion Erythrocyte transfusion

Interventions

Screening All subjects, healthy volunteers aged 18-35 will be screened at the AMC and at Sanquin. Investigation of the medical history, a physical examination, ECG and blood and urine examination wi

miniature whole blood donation processed with a whole blood leukodepletion filter

validated Sanquin product) which will be processed into a small RBC concentrate. Both donations will be labelled with biotin, using two densities of biotin according to previously published protoco

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: 1) Healthy volunteer 2) Age * 18 years

Exclusion criteria

Exclusion criteria: 1) No informed consent 2) Any abnormal test result during the screening prior to inclusion of the study (medical history, physical examination, ECG, blood and urine examination) 3) History of drugs or alcohol abuse 4) Any present medication use on prescription 5) Smoking

Design outcomes

Primary

Measure	Time frame
1) Compare post-transfusion RBC clearance of fresh and stored RBCs and investigate whether glycolytic enzyme function, glycolysis, pentose phosphate pathway function and related metabolic pathways recover after transfusion of a 35 day stored RBC product in humans.	—

Secondary

Measure	Time frame
1) Glycolytic enzyme function, glycolysis, pentose phosphate pathway function and related metabolic pathways recovery after transfusion of stored RBCs compared to fresh RBCs. 2) Glycolytic enzyme function, glycolysis, pentose phosphate pathway function and related metabolic pathways function during storage of RBCs in PAGGGM or a similar alkaline additive solution compared to RBCs in SAGM. 3) Glycolytic enzyme function, glycolysis, pentose phosphate pathway function and related metabolic pathways recovery after transfusion of stored RBCs in PAGGGM or a similar alkaline additive solution compared to RBCs in SAGM.	—

Measure

Time frame

1) Glycolytic enzyme function, glycolysis, pentose phosphate pathway function and related metabolic pathways recovery after transfusion of stored RBCs compared to fresh RBCs. 2) Glycolytic enzyme function, glycolysis, pentose phosphate pathway function and related metabolic pathways function during storage of RBCs in PAGGGM or a similar alkaline additive solution compared to RBCs in SAGM. 3) Glycolytic enzyme function, glycolysis, pentose phosphate pathway function and related metabolic pathways recovery after transfusion of stored RBCs in PAGGGM or a similar alkaline additive solution compared to RBCs in SAGM.

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Countries

Netherlands

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)