composition of intestinal microbiota development of allergy
Conditions
Interventions
None listed
Sponsors
Sint Antonius Ziekenhuis
Eligibility
Age
2 Years to 11 Years
Inclusion criteria
Inclusion criteria: Term born babies (i.e. > 36 weeks gestational age) (Short) stay on maternal ward OR admission to neonatal ward because of antibiotic treatment Signed informed consent by the parents
Exclusion criteria
Exclusion criteria: Prematurity (i.e. gestational age
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary endpoint of the study is the incidence of eczema in antibiotic treated children versus healthy controls. Besides that, the incidence of food allergy, URTI, LRTI and GITI will be monitored. This will illustrate whether early antibiotic treatment may influence the developing immune system. Objective evaluation will be done by measuring vaccination response, length and weight (weight/growth curves) and recall doctor*s diagnoses from the GP (primary care visits for URTI, LRTI, GITI, food allergy, atopic dermatitis). Even so, usage of medication will be determined by checking pharmacist*s medication records and by asking the parents to report antibiotic treatment of their child on the monthly checklists. | — |
Secondary
| Measure | Time frame |
|---|---|
| The secundary outcomes of the study are potential differences in the development of intestinal microbiota (i.e. microbiota composition) in infants exposed to antibiotic therapy in early life (first 2 weeks) versus infants that did not receive antibiotic treatment during that period. The samples are subjected to microbiota composition profiling by means of a PCR-based interspace profiling technique (IS-pro, IS-Disgnostics, Amsterdam). Composition of faecal samples will be analyzed by targeting bacterial groups that are present (e.g. Bifidobacteria, Enterobacteriaceae, Streptococci, Lactobacilli, Bacteroides, Proteobacteria) and by pyrosequencing technique at the University of Wageningen (head Prof. J. Knol). . | — |
Countries
Netherlands
Outcome results
None listed