metabolisme van vetten Lipid metabolism
Conditions
Interventions
The subjects of this study will receive, at study days 1-4 and 10, a high-fat
drink, which will be a dairy drink consisting of full cow*s milk, cream, skim
milk powder and lactose (total of 10% fat)
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: * Caucasian male * 18-35 years old * Body mass index (BMI) 22-25 kg/m2. * Fat percentage between 8 * 15% * Regular consumption of milk (products)
Exclusion criteria
Exclusion criteria: * (symptoms of) cow*s milk allergy * Lactose intolerance * Metabolic diseases * (known symptoms of) (auto)immune diseases, like diabetes * (known symptoms of) intestinal diseases, like; irritable bowel syndrome Intestinal malabsorption, diagnosed with celiac disease, Crohn*s disease, colitis ulcerosa, short bowel syndrome or surgical bowel interventions leading to malabsorption; * Usage of medication * Usage of hard drugs * History of smoking * Claustrophobia * Unsuitable veins for blood sampling * Blood donation during the two months before the start of the study * Current participation in other scientific studies
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main study objective is the postprandial change in VOCs from exhaled air. This parameter will be studied to gain insight in the the intra-subject variation of VOC profile in exhaled air in a fasted state and after consumption of a low- or high-fat drink. | — |
Secondary
| Measure | Time frame |
|---|---|
| The secondary study objectives are parameters (which will be investigated during part II of the study) to investigate whether specific VOCs in exhaled air after fat consumption can be linked to postprandial VOCs in blood; and to investigate whether specific VOCs in exhaled air after fat consumption can be linked to postprandial (metabolic) parameters. Therefore, the secondary parameters are: * Postprandial change in VOCs from blood samples * Blood levels: as indication of fat absorption and metabolism o Plasma lipid profile over 5 hours after consumption of milk drinks (level of free fatty acids (FFA), level of LDL, HDL and VLDL, total cholesterol, triglycerides and cholesterol in chylomicron-rich fractions and size of those fractions) o Apolipoprotein B48 o Apolipoprotein A-IV o Glycated haemoglobin (only at baseline) o Etc. * Respiratory exchange ratio (RER) (ratio of CO2 production and O2 consumption). The CO2 produced and O2 consumed by participants will be measured in resting condition, pre- and 5 hours postprandial with the usage of a ventilated hood system (Max II Metabolic Cart, AEI technologies). | — |
Countries
Netherlands
Outcome results
None listed