vermogen tot herstel van lichte spierschade en adaptatie aan inspanning en training bij gezonde personen mild muscle damage due to normal exercise and training
Conditions
Interventions
exercise test
gene expression
healthy athletes
RNA-profiles
Sponsors
Stichting Hogeschool Leiden
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: - Healthy males -18-50 years old - Recreationally trained track or road cyclists (VO2-max >= 45ml/kg/min), with at least two years of cycling experience of at least twice a week - Meeting criteria of a valid maximal incremental exercise test - Body mass index (BMI) 20-25 kg/m2 - Veins suitable for blood sampling at inspection - Based on the outcome of the anamnesis form, participants should be rated as low risk participants
Exclusion criteria
Exclusion criteria: Men with health problems and/or without sporting activity or above 50 years old - Known diagnosis of immune disease such as diabetes, coeliac disease, rheumatoid arthritis - Known diagnosis of gastro-intestinal disease such as Crohn*s disease, colitis ulcerosa, irritable bowel syndrome -Smoking -Use of hard drugs -Use of specific medicines: *chronic use of NSAIDs: aspirin, ibuprofen, corticosteroids *chronic use of antidepressiva, antacids (Rennie), benzodiazepines (Valium * drugs against abdominal pain and cramping (e.g.buscopan, imodium), -Participation in other scientific studies within 1 month before the preliminary testing
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| RNA-profiles of blood cells, as indication of functional alterations in gene expression in leukocytes. Exercise performance parameters as described in the model by Joyner and Coyle (2008) as determined measured during the maximal incremental exercise tests at the start and end of the project: Performance VO2 - VO2max, VO2 at the ventilatory threshold, performance O2 deficit, gross efficiency, peak power output and average power output attained during a Wingate test. | — |
Secondary
| Measure | Time frame |
|---|---|
| The secundary objectives are: 1) To assess the correlation between changes in gene expression patterns and changes in exercise performance in individuals to determine the effects of training during and after a regular training period of approximately 12 weeks. The secondary study parameters will be the same as the primary endpoints. 2) To assess the correlation between changes in gene expression patterns with changes in several physiological performance-determining variables and changes in metabolites. In the model described by Joyner and Coyle (7) four main physiological parameters are considered to be important for exercise performance: 1) VO2max, 2) VO2 at the lactate or ventilatory threshold, 3) anaerobic capacity (performance O2 deficit), and 4) GE. Therefore, secondary study parameters are related to these four physiological parameters. 3) To investigate the suitability of small blood samples obtained from finger pricks for the generation of gene expression patterns. The quality of RNA isolated from finger prick and venous blood samples will be compared. When sufficient, RNA profiles will be generated from both sources. These will be compared to determine if finger prick profiles can be used to detect similar effect sizes and adequately monitor changes in gene expression. 4) To select and test a set of differentially expressed genes for the development of a specific test to monitor training effects. RNA-Seq is a sensitive technique, but time-consuming and still expensive. To effectively monitor training effects, a specific test based on a subset of genes which are differentially expressed under the study conditions would be required. To develop a specific test a set of differentially expressed genes selected in the pilot study will be validated by quantitative RT-PCR in the main study. | — |
Countries
The Netherlands
Outcome results
None listed