Immuun respons na inspanning Exercise induced immune response
Conditions
Interventions
The intervention consists of a custom diet that is followed for 2 weeks. After
2 days on the diet, a test day takes place and after 2 weeks on the diet
another test day takes place. The diet is low,
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Male Age: 18 - 45 y BMI: 18.5 * 25 kg/m2 Hb * 8.5 mmol/L, determined with blood sample from finger tip Minimal of 3 hours of training per week, and a maximum of 10 hours DKTP (diphtheria, pertussis, tetanus and polio) vaccinated (for stimulation of isolated cells (PBMC*s) with tatanus toxoid) No asthma medication and/or anti-inflammatory medication No use of immunosuppressive medication No flu and/or travel vaccinations in the 4 months before and during the study Able to be present and participate at all test days Willing and able to follow prescribed diet for 2 weeks in a row (2 times 2 weeks)
Exclusion criteria
Exclusion criteria: Chronic illness Blood donations during study or in 2 months prior to the study Food allergies (e.g. nuts, gluten, avocado) Vegetarian diet Use of immunosuppressive medication Flu and/or travel vaccination 4 months before and during the study Hb
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Blood sample: o Serum/plasma: cytokines, CRP, cortisol o *Homing* estimations: where do the immune cells move to? To upper respiratory tract or to intestines? (*4*1/CCR10/CD62L for upper respiratory tract) (*4*7/ CCR9 for intestines). o PBMC*s (peripheral blood mononuclear cells): stimulate with LPS and measure IL-1, TNF, IL-6, IL-10 and IL-12 o PBMC*s (peripheral blood mononuclear cells): stimulate with Tetanus Toxoid (TT) o PBMC*s (peripheral blood mononuclear cells): stimulate with conA (lectine) as positive control o PBMC*s (peripheral blood mononuclear cells): stimulate with Pokeweed as postive control for B-cells. o PBMC*s (peripheral blood mononuclear cells): Estimate proliferation by adding K167. | — |
Secondary
| Measure | Time frame |
|---|---|
| - Faeces sample: intestine immunity (with Calprotectin test) - Saliva sample: IgA and TNF-alpha (with ELISA test) - Urine sample: Ketone bodies with Ketostix - Blood sample: stress hormones, glucose, free fatty acids, ketone bodies. Tertiary: Blood: lactate, insulin, GI permeability markers Body composition: weight, length, fat percentage, bone and muscle weight (DEXA) Diet: food questionnaire app Physical activity: Physical activity level-questionnaire Questionnaires: delayed onset muscle soreness (DOMS), upper respiratory tract infection (URTI) questionnaire. Motivational Q and questionnaire on sleep, mental physical and emotional wellbeing Performance: RER (ratio CO2:O2) during exercise test. | — |
Countries
Netherlands
Outcome results
None listed