Spastic colon
Conditions
Interventions
None listed
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: For IBS patients: - Adults, aged 18-65 years. - IBS patients that meet the Rome IV criteria. - In close proximity of Wageningen (max. 50 km), for practical reasons: collection of the faecal samples. - Have an Body Mass Index (BMI) between 18.5 and 30 kg/m2. - Signed informed consent. - After T1, IBS severity will be checked using the IBS-SSS. Subjects will be included for follow-up at T2, when they have the most mild symptoms (n
Exclusion criteria
Exclusion criteria: For IBS patients: Presence of gastro-intestinal diseases, such as celiac disease, Crohn*s disease, or Ulcerative colitis. - Have a history of intestinal surgery that might interfere with study outcomes. This does not include an appendectomy or cholecystectomy. - Presence of significant systemic diseases, such as diabetes mellitus, cancer, cardiovascular disease or respiratory disease. - Female participants: currently pregnant or breast-feeding. - Use of antibiotic treatment less than 3 months before start of the study and no use of antibiotics during the study. - Are not participanting in another study during this study. - Are an employee or student of the Division of Human Nutrition, Food and Biobased Research or Laboratory of Microbiologie, of Wageningen University & Research. - Unwilling or unable to fulfil study criteria. - If they are not selected in the most mild or most severe group at T1. ;For healthy controls: - Presence of gastro-intestinal diseases, such as celiac disease, Crohn*s disease, or Ulcerative colitis. - Have a history of intestinal surgery that might interfere with study outcomes. This does not include an appendectomy or cholecystectomy. - Presence of significant systemic diseases, such as diabetes mellitus, cancer, cardiovascular disease or respiratory disease. - Female subjects: currently pregnant or breast-feeding. - Use of antibiotic treatment less than 3 months before start of the study. - Are an employee of the Division of Human Nutrition, Food and Biobased Research or Laboratory of Microbiology, of Wageningen University & Research. - Are subjects in another study during this study. - Unwilling or unable to fulfil study criteria.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Primary endpoint of this study is microbiota composition and metabolite profiles, and the difference between groups and possible change after one month. These are assessed two faecal samples, which are analysed by 16S rRNA gene-based microbiota profiling. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary parameters are dietary intake, Quality of Life, depression and anxiety scores, and stool consistency and frequency, which are assessed by validated questionnaires. | — |
Countries
Netherlands
Outcome results
None listed