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Modelling of human skin diseases using in vitro 3D reconstructed skin

Modelling of human skin diseases using in vitro 3D reconstructed skin - 3D models of human skin diseases

Status
Unknown
Phases
Unknown
Study type
Observational
Source
NL-OMON
Registry ID
NL-OMON46406
Enrollment
113
Registered
2018-07-17
Start date
Unknown
Completion date
Unknown
Last updated
2024-04-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

inflammatory skin diseases (atopic dermatitis and psoriasis) and genodermatoses (skin diseases caused by genetic disorder)

Interventions

None listed

Sponsors

Radboud Universitair Medisch Centrum
Lead Sponsor

Eligibility

Age
18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Patients that suffer from atopic dermatitis, psoriasis and genodermatoses

Exclusion criteria

Exclusion criteria: If it is likely that patients develop hypertrophic scars (patients are asked if they do/don't heal nicely after damage to the skin).

Design outcomes

Primary

MeasureTime frame
* The outcome measures of the cell culture experiments include: morphology of the epidermis and gene expression of the epidermal cells, following stimulation of the cells (e.g. by cytokines or microbes). Gene expression analysis will generate quantitative data (delta Ct of qPCR). * Histology will be scored semi quantitatively (stain intensity and localization). * We will identify relevant mutations of risk genes for psoriasis and AD. In case of genodermatoses, the causative genes are in general already known. * The effect of diagnosis and genotype on gene expression and histology will be analysed with multivariate techniques.

Secondary

MeasureTime frame
None

Countries

Netherlands

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)