Endothelial Colony Forming Cells, Endothelial Progenitor Cells and Bicuspid Aortic Valves: in vitro culture of peripheral blood cells to study biomarkers and pathogenesis in BAV?

Endothelial Colony Forming Cells, Endothelial Progenitor Cells and Bicuspid Aortic Valves: in vitro culture of peripheral blood cells to study biomarkers and pathogenesis in BAV? - In vitro endothelial cell modelling of patients with bicuspid aortic valves

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON46125

Enrollment

400

Registered

2016-06-02

Start date

2017-06-02

Completion date

Unknown

Last updated

2024-04-23

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Bicuspid aortic valves two aortic valves

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: • aged >= 18 years; • capable of understanding and signing informed consent. • echocardiogram at most 1 year and 2 months before blood drawing • for the patient population: Bicuspid aortic valves

Exclusion criteria

Exclusion criteria: • Consuming more than 3 units of alcohol the evening before drawing blood For the control population: • Valvular stenosis • Regurgitation • Aortic dilation

Design outcomes

Primary

Measure	Time frame
The parameters studied are endothelial cell communication and endothelial cell function. - - The cell communication will be primarily studied in the ECFCs. The response of smooth muscle cells to the presence of ECFC secreted factors will determine what the focus will be of the study. There are 4 likely candidates: inflammatory factors, growth factors, nitric oxide regulation and extracellular matrix remodelling. - Endothelial cell function will be studied in the EMT response, migration and calcification. - The results from the ECFCs will then be studied in the EPC*s to look for potential biomarkers	—

Measure

Time frame

The parameters studied are endothelial cell communication and endothelial cell function. - - The cell communication will be primarily studied in the ECFCs. The response of smooth muscle cells to the presence of ECFC secreted factors will determine what the focus will be of the study. There are 4 likely candidates: inflammatory factors, growth factors, nitric oxide regulation and extracellular matrix remodelling. - Endothelial cell function will be studied in the EMT response, migration and calcification. - The results from the ECFCs will then be studied in the EPC*s to look for potential biomarkers

—

Secondary

Measure	Time frame
The results obtained with the ECFC*s will studied for potential links to valvular development. This might elucidate the pathogenesis of bicuspid aortic valves.	—

Countries

Netherlands

Outcome results