contactallergy / contactdermatitis
Conditions
Interventions
Two skin biopsies will be taken from each case, one skin biopsy from each
healthy control. For cases, the biopsies will be taken from the skin area on
the volar side of both forearms, where on one a
Sponsors
Universitair Medisch Centrum Groningen
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Case group: - A proven contact allergy (by previous patch testing at the department of dermatology in the UMCG) to either/both FM I and/or FM II. - Caucasian female - Age between 20 and 50 years - Legal competence;Control group: - Caucasian female - Age between 20 and 50 years - No clinical history of dermatitis reaction to fragranced products - Legal competence
Exclusion criteria
Exclusion criteria: - Skin-anomalies on the forearm such as active eczema - Current atopic dermatitis or psoriasis - The use of immunosuppressive medication during the study or in the four weeks before inclusion (oral corticosteroids, cyclosporine, methotrexate, azathioprine and biologicals) - Legally incompetent - Difficulty understanding spoken and written information in Dutch.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Keratinocytes from the biopsies of allergic individuals and controls will be isolated and propagated in vitro. They will be cocultured with the dendritic cell surrogate THP-1 cells and exposed to purified terpene hydroperoxides. As main study parameters/endpoints will be the release of inflammatory mediators and expression of activation markers in and on both types. | — |
Countries
Netherlands
Outcome results
None listed