Neutrophil Inhibition during RSV bronchiolitis and other respiratory tract infections

Neutrophil Inhibition during RSV bronchiolitis and other respiratory tract infections - Neon study

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON45612

Enrollment

175

Registered

2016-09-21

Start date

2016-11-16

Completion date

Unknown

Last updated

2025-08-18

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

bronchiolitis respiratory tract infections

Interventions

None listed

Eligibility

Age

2 Years to 11 Years

Inclusion criteria

Inclusion criteria: Group 1: RSV, mechanical ventilation (n

Exclusion criteria

Exclusion criteria: Severe comorbidity, such as any organ dysfunction except for cardiac malformalities or the presence of a trachea canulue. Immune suppressed patients such as patient using immune suppressives or receiving chemotherapy. Any severe congenital or acquired abnormality, such as Down syndrome

Design outcomes

Primary

Measure	Time frame
Flow cytometry of peripheral blood will be used to determine expression of inhibitory receptors in peripheral blood cells of patients. Neutrophil function regulation by inhibitory receptors in tracheal aspirate cells will be studied. ELISA will be used to determine levels of soluble inhibitory receptors and other serum cytokines.	—

Secondary

Measure	Time frame
RSV staining of epithelial cells and qPCR on cell adhesion and epithelium-mesenchymal transition (EMT) genes will be used to study interaction between epithelium and leukocytes. In vitro culture of epithelial cells and autologous blood neutrophil, macrophages or lymphocytes to study various leukocyte functions.	—

Countries

Netherlands

Outcome results