Mitochondrial Capacity in Untrained and Endurance-trained Young Males

Mitochondrial Capacity in Untrained and Endurance-trained Young Males - MCAP

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON45254

Enrollment

Registered

2017-05-13

Start date

2017-09-17

Completion date

Unknown

Last updated

2025-03-24

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Veroudering Ageing Sarcopenia

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: 18-28 year old male BMI 18,5-25 kg/m2 VO2max * 47 mL/kg/min or VO2max * 57 mL/kg/min

Exclusion criteria

Exclusion criteria: - Health concerns regarding respiratory and pulmonary diseases, such as COPD, (exercise induced) asthma and cardiovascular disease. - (known symptoms of) Metabolic diseases, such as type I or II diabetes, - Regular smoker (defined as smoking >5 cigarettes per week) - Haemoglobin concentrations below 8.0 mmol/L - Recent usage (within four months) of supplements with suggestive training effects, such as creatine phosphate, EPO or anabolic steroids. - Usage of recreational drugs, such as marihuana, amphetamines and cocaine during the study (starting after screening day) - Suffers from (sport) injury that hampers maximal exercise performance - Blood donation during the course of study - Current participation in other clinical trials - Employed or undertaking a thesis or internship at the department of Human and Animal Physiology

Design outcomes

Primary

Measure	Time frame
The main study parameter is the rate of recovery of muscle oxygen consumption measured using NIRS combined with transient arterial occlusions. The rate of recovery is expressed as a time constant. Subjects will be screened for VO2max, measured using an incremental exercise protocol on a bicycle ergometer. Eight endurance-trained and eight untrained subjects will be included based on their VO2max. These subjects are invited to a study test day, in which they are asked to arrive in a fasted state for a blood sample . Afterwards mitochondrial capacity will be measured in the gastrocnemius and wrist flexor muscles using transient arterial occlusions and NIRS measurements.	—

Measure

Time frame

The main study parameter is the rate of recovery of muscle oxygen consumption measured using NIRS combined with transient arterial occlusions. The rate of recovery is expressed as a time constant. Subjects will be screened for VO2max, measured using an incremental exercise protocol on a bicycle ergometer. Eight endurance-trained and eight untrained subjects will be included based on their VO2max. These subjects are invited to a study test day, in which they are asked to arrive in a fasted state for a blood sample . Afterwards mitochondrial capacity will be measured in the gastrocnemius and wrist flexor muscles using transient arterial occlusions and NIRS measurements.

—

Secondary

Measure	Time frame
EPOC will be measured following a short sub-maximal exercise bout on a bicycle ergometer by indirect calorimetry. Mitochondrial function will be measured in PBMC*s extracted from blood using the Seahorse XFe analyzer.	—

Countries

Netherlands

Outcome results