Inactivity Skeletal Muscle Disuse
Conditions
Interventions
Applying blood flow restriction during 2 weeks of bed rest.
Blood flow restriction
Inactivity
Muscle
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: Healthy males Age between 18 and 35 y BMI between 18.5 and 30 kg/m2
Exclusion criteria
Exclusion criteria: -Smoking -Type 2 Diabetes Mellitus -Any back/leg/knee/neck/postural complaints * -Any history or family history of thrombosis* -All co-morbidities interacting with mobility and muscle metabolism of the lower limbs *(e.g. arthritis, spasticity/rigidity, all neurological disorders and paralysis) -Myocardial infarction within the last 3 years -Use of certain anti-coagulants (use of thrombocyte aggregation inhibitors such as Ascal, *acetylsalicylic acid, aspirin and carbasalaatcalcium is permitted. Use of other thrombocyte aggregation inhibitors will be discussed with the responsible physician) * -Performing regular resistance training (3+ times per week, carrying out progressive training) in the previous 6 months -Hypertension (according to WHO criteria) and/or cardiovascular disease -A history of deep vein thrombosis (DVT) in the leg -Having donated blood in the 3 months prior to the study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary outcome measure is the amount of muscle mass in the legs before and after 2 weeks of bed rest with the application of blood flow restriction. | — |
Secondary
| Measure | Time frame |
|---|---|
| Whole-body skeletal muscle mass Leg and whole-body lean tissue mass Quadriceps muscle CSA Muscle fibre type specific CSA, vascularization and muscle fibre type-specific SC content Myofibrillar and mitochondrial fractional synthesis rate Whole-body adipose tissue Adipose tissue gene expression Leg muscle and maximal grip strength Oral Glucose Tolerance | — |
Countries
The Netherlands
Outcome results
None listed