asthmatic bronchitis Bronchitis
Conditions
Interventions
None listed
Sponsors
Universitair Medisch Centrum Groningen
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Inclusion criteria for all subjects: • Age between 40 and 65 years old. • Smoking history 90% predicted. • Absence of bronchial hyperresponsiveness, i.e. both PC20 methacholine > 8 mg/ml and PC20 AMP > 320 mg/ml.;o Group 3. Patients with ongoing asthma • Documented history of asthma diagnosed according to latest GINA guidelines, i.e. respiratory symptoms and either bronchodilator reversibility (improvement in FEV1 of more than 12% of baseline (and at least 200 mL) after inhalation of 800 µg salbutamol. • Use of inhaled corticosteroids or Either persistent symptoms of wheeze, cough, or dyspnea or regular use of β2 agonists at least once a week during the last 2 months. • PC20 methacholine 8 mg/ml, FEV1/FVC > 70% and FEV1 > 80% predicted.
Exclusion criteria
Exclusion criteria: • FEV1
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary endparameter will be the difference in the transcriptomic profile of innate lymphocytes, CD4+ T cells, and CD8+ T cells and epithelial cells in bronchial biopsies and of blood eosinophils obtained from subjects with ongoing asthma, clinical or complete asthma remission and healthy controls. | — |
Secondary
| Measure | Time frame |
|---|---|
| Questionnaires Questionnaires will be the ELON questionnaire, the Asthma Control Questionnaire (ACQ), and the small airways dysfunction tool (SADT). Spirometry measurements: FEV1, FVC, FEF25, FEF50, FEF75 and FEF25-75. Body plethysmography: TLC, RV, FRC, VC, IVC. Specific airway conductance (sGaw), airway resistance (Raw). Impulse oscillometry (IOS): Parameters assessed with this test are: R5, R20, X5, R5-R20. Skin prick test for allergy Peripheral blood • Hemoglobin, leucocytes and differentiation, thrombocytes (10 mL peripheral blood; EDTA tube). • DNA and plasma (10 mL peripheral blood; EDTA tube). • RNA (10 mL, PAXgene tube) • Serum (10 mL peripheral blood serum tube). • Total IgE, Phadia-top and CRP will be measured in serum (10 mL peripheral blood serum tube). • PBMCs (40mL peripheral blood; heparin lithium tubes). Methacholine and Adenosine Monophosphate (AMP) provocation HRCT scan of the thorax Multiple Breath Nitrogen Washout (MBNW): Parameters assessed are LCI, Sacin, Scond. Exhaled Nitric Oxide (single breath at multiple flows) PExA: Particles in exhaled air. Inflammatory cell counts in bronchial biopsies and induced sputum. mRNA, non-coding small and large RNA, and DNA methylation in bronchial and brushes, nasal epithelial brushes. | — |
Countries
Netherlands
Outcome results
None listed