Obesity overweight/fatness
Conditions
Interventions
The intervention consists of a hypercaloric (60% caloric excess) diet, during a
period of approximately 30 days, followed by a hypocaloric phase (1.0 x resting
energy expenditure), until subjects re
Sponsors
Vrije Universiteit Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: 1. Age 18*30 years 2. Male gender 3. Caucasian 4. BMI 20*25 kg/m2 5. Normal insulin sensitivity 6. Normoglycemia at time of screening 7. Normal diet pattern 8. Stable body weight during 6 months before enrolment in the study (
Exclusion criteria
Exclusion criteria: 1. Presence of any relevant disease 2. Use of any relevant medication 3. First-degree relative with type 2 diabetes 4. Smoking 5. Shift work 6. A history of chronic glucocorticoids use or GC use
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Metabolic insulin sensitivity: rate of disappaerance (Rd) en endogenous glucose production (EGP) 2. Vascular insuline senitivityt: changes in microvascular blood volume (MBV) | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Microvascular vasoreactivity in vitro with and without PVAT (myograph technique) 2. Insulin mediated protein expression and activation in microvessels, adipose and skeletal muscle tissue 3. Gen expression of insulin signaling genes in endothelial cells 4. PVAT morphology/characteristics, including adipocyte size, celltypes and expression of adipokines 5. Plasma insulin, glucose, lipid spectrum, inflammatory parameters 6. Physical activity (by means of accelerometry) 7. Gut microbiota composition (fecal analysis) | — |
Countries
Netherlands
Outcome results
None listed