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Clinical validation of a dried blood spot (DBS) method for the analysis of immunosuppressive and antifungal drugs in pediatric patients (part of the PROTECT study).

Clinical validation of a dried blood spot (DBS) method for the analysis of immunosuppressive and antifungal drugs in pediatric patients (part of the PROTECT study). - PROTECT

Status
Active, not recruiting
Phases
Unknown
Study type
Observational
Source
NL-OMON
Registry ID
NL-OMON44369
Enrollment
126
Registered
2014-11-24
Start date
2015-05-12
Completion date
Unknown
Last updated
2024-04-23

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

immuunsuppressie na niertransplantatie analysis of drug used in renal transplantation / analysis of drug used in invasive fungal infections

Interventions

None listed

Sponsors

Afdeling Apotheek
Lead Sponsor

Eligibility

Age
2 Years to 17 Years

Inclusion criteria

Inclusion criteria: • Patients aged between 2 and 18 years • Admitted to the pediatric ward or visiting the doctor on an outpatient basis • Having a venous catheter or blood is drawn for regular patient care • Treated with at least 1 of the 9 drugs of interest • Signed informed consent

Exclusion criteria

Exclusion criteria: • Parents and/or patients are not able to understand the Dutch language

Design outcomes

Primary

MeasureTime frame
The primary objective of this study is the clinical validation of a DBS method for voriconazole, fluconazole, itraconazole, posaconazole, mofetil mycophenolic acid, cyclosporine, tacrolimus, sirolimus and everolimus in the pediatric population. The related endpoint will is the evaluation ofbe the association between the concentration obtained by venous sampling and the concentration obtained by means of DBS sampling. The predictive performance of the DBS method as a measure for the venous concentration will be evaluated.

Secondary

MeasureTime frame
- To assess the feasibility of finger prick DBS in the pediatric population. The related endpoint is the response to a questionnaire. Results will be used to prepare implementation of the novel method for home-based monitoring as well as to prepare a HTA analysis. - To design an inventory of cost types related to DBS sampling and conventional sampling. The cost types will function as a basis for future HTA analysis of this novel sampling method compared to conventional venous sampling. - To use the date already collected for the primary endpoint to construct a population pharmacokinetic model to optimize dosing and design new guidelines of the nine compounds included in this study (voriconazole, fluconazole, itraconazole, posaconazole, mofetil mycophenolic acid, cyclosporine, tacrolimus, sirolimus and everolimus) for the pediatric patient population. Endpoints will be population estimates of the pharmacokinetic parameters AUC, maximal concentration (Cmax), time to maximal concentration (Tmax), clearance (CL), volume of distribution (Vd) and elimination half-life (t1/2).

Countries

Netherlands

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)