Verteringskinetiek van eiwit bij gezonde mensen NVT
Conditions
Interventions
Subjects come to the research facility in fasting state. After consuming the
soup (~550 ml with 20g of protein), blood samples will be taken each 15 minutes
for a period of three hours to assess the
amino acid profile
plant-based protein
post-prandial effects
Sponsors
Stichting Wageningen Research -- Wageningen Food & Biobased Research
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: * Healthy as assessed by a health and lifestyle questionnaire and normal blood clinical laboratory tests for haemoglobin, kidney and liver functioning * Age between 18 and 50 y (boundaries included) at day 01 of the study * Body mass index (BMI) between 19 and 25 kg/m2 (boundaries included) * Appropriate veins for blood sampling (judged by a study nurse or medical doctor) * Willing to abstain from blood donation one month before and during the study * Willing to abstain from the use of protein supplements during the study period * Signed informed consent * Willing to comply with the study procedures
Exclusion criteria
Exclusion criteria: * Any metabolic, gastrointestinal, inflammatory or chronic disease (such as diabetes, anaemia, hepatitis, cardiovascular disease) * History of gastro-intestinal surgery * History of liver dysfunction (cirrhosis, hepatitis) or liver surgery * Kidney dysfynction (eGFR 16 hours per week) * Current smoker * Alcohol intake *3 glasses of alcoholic beverages per day * Pregnant or lactating or wishing to become pregnant in the period of the study (self-reported) * Use of hard drugs * Known allergies towards the products used in the study * Not willing to consume chicken bouillon * Participation in another clinical trial at the same time * Being an employee of the department of Human Nutrition of Wageningen University or the Consumer Science & Health group of Wageningen Food & Biobased Research
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main study parameter is the peak value and total amount of amino acids measured in the serum before (t=0 min) and at the various time points after consumption of the two proteins (until t=180 min). | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary outcomes are the glucose and insulin levels in the blood samples in the same time period of three hours (t=0; t=15, t=30 * t=180) after consumption of the two protein products. Aural temperature, heart rate and blood pressure will be measured before (t=0 min) and after consumption (t=180 min). Finally, subjects will report gastro-intestinal complaints on the test day (consumption of the protein soup) and the subsequent three days via a diary. | — |
Countries
The Netherlands
Outcome results
None listed