community of microorganisms microbiome
Conditions
Interventions
None listed
Sponsors
Medisch Universitair Ziekenhuis Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: * Diagnosis of COPD stages I-IV, class A-D, as defined by the Global initiative for chronic Obstructive Lung Disease (GOLD); * Patients must be planned to undergo a bronchoscopy; * Patients must be able to complete questionnaires; * Patients must sign and date an informed consent prior to inclusion.
Exclusion criteria
Exclusion criteria: * Chronic use of oral corticosteroids > 10 mg/day; * Investigator*s uncertainty about the willingness or ability of the patient to comply with the protocol requirements, e.g. not smoking 6 hours before and fasting two hours before sputum induction; * Patients with mental conditions rendering them unable to understand the nature, scope, and possible consequences of the study; * Patients unlikely to comply with the protocol, e.g. uncooperative attitude, and unlikelihood of completing the study (not able to attend all three visits).
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To determine the optimal sample type for respiratory microbiome analysis using the IS-pro technology. | — |
Secondary
| Measure | Time frame |
|---|---|
| - Intra-patient analysis to define and compare the respiratory microbiome and its microbial diversity by BAL fluid, bronchial aspirate, induced sputum samples, throat and nose swabs; - Longitudinal analysis of respiratory microbiome in sputum, throat and nose swabs, to assess the respiratory microbiome and the microbial diversity of patients over time, between the same and different sample types. | — |
Countries
Netherlands
Outcome results
None listed