Lipid metabolism obesity
Conditions
Interventions
A single night of short sleep duration (4 hours) compared to a control
condition of normal sleep duration (8 hours).
Sponsors
Leids Universitair Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Informed consent - Caucasian men - Age between 18 and 55 years - BMI between 20 and 25 kg/m2 (equally divided over the range, so ideally n=12 in BMI categories 20 -25; 25 - 30; 30 - 35 kg/m2)
Exclusion criteria
Exclusion criteria: Active endocrine disease (e.g. diabetes mellitus type 1 and type 2, thyroid disease, Cushing*s disease and lipid-associated disorders such as FH) - Fasting glucose >7.0 mmol/L - Severe chronic disease (e.g. chronic liver or kidney disease) - Severe insomnia, sleep disorders or exceptional habitual sleep duration (10 h). - Medication use including the following: lipid lowering drugs, glucocorticoids, sleep medication, hormone replacement, glucose lowering drugs, insulin therapy (last 6 weeks), anticoagulants - Recent time zone travel (last 6 weeks) - Shift work (last 6 weeks) - Severe alcohol use (>21 units/week) - Psychiatric disease - Drug abuse - Recent participation to another nutritional or biomedical trial (last 6 weeks) - Taking medication, which may interfere with study measurements, as judged by the responsible physician - Reported weight loss or weight gain (10%) in the last six months prior to the pre-study screening - Clinically relevant abnormalities in clinical chemistry at screening (to be judged by the study physician) - Reported use of any nicotine containing products in the six months preceding the study and during the study itself; - Extreme strenuous exercise during last 3 months, as judged by responsible physician - Excessive sunbathing during the last 3 months, as judged by responsible physician
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primairy study parameters are: - Lipid metabolism by measurement of hormone and lipid concentrations in plasma in response to a mixed-meal challenge - Energy metabolism by measurement of indirect calorimetry - HPA-axis responsiveness by hormone and blood pressure measurement during a cold- feet pressor test - Mood via short questionnaires (Profile of Mood States) | — |
Secondary
| Measure | Time frame |
|---|---|
| The secondairy study parameters are: - Gene expression profiles and metabolomics in muscle and fat tissue and plasma - Gene expression profiles in skin and hair samples | — |
Countries
Netherlands
Outcome results
None listed