ELISA to measure urinary exosomal content: proof of principle study

ELISA to measure urinary exosomal content: proof of principle study - Measuring protein content of urine vesicles

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

NL-OMON

Registry ID

NL-OMON43613

Enrollment

Registered

2017-08-24

Start date

2015-10-01

Completion date

Unknown

Last updated

2024-02-28

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Fysiologische studie - testen van methodiek N.A.

Interventions

14 hour fasting and water loading (20 mL/kg body weight in 30 minutes)

ELISA

Exosomes

Urine

Eligibility

Age

18 Years to 64 Years

Inclusion criteria

Inclusion criteria: Have the capacity to understand and willingness to sign an informed consent form. Aged 18-30 years

Exclusion criteria

Exclusion criteria: - Use of medication - History of diabetes insipidus, diabetes mellitus, adrenal deficiency, thyroid disease or chronic kidney disease - Alcohol abuse - Urinary tract infection or menstruation at the time of inclusion - Pregnancy or breast feeding

Design outcomes

Primary

Measure	Time frame
The primary outcome will be a change in urine osmolality after fasting and water loading.	—

Secondary

Measure	Time frame
Change of urine exosomal aquaporin-2 and NCC between fasting and after water loading. Furthermore the number of excreted exosomes.	—

Countries

The Netherlands

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)