geen, methodologie methodology non
Conditions
Interventions
On two separate test days, subjects will receive a semi liquid meal (pudding)
divided in 9 portions, (500 Kcal
meal 1: 27 En% Protein,10 En% fat, 62 En%
carbohydrates
meal 2: 14 En% Protein,10 En% fat, 75 En% carbohydrates), made
with intrinsically labelled 15N protein powder. Furthermore, the meal will
contain a trace amount of 13C-labelled algae protein as an i
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: * Healthy * Age: 20 * 35 y * BMI: 18.5 * 25 kg/m2 * Stable dietary habits * Veins suitable for cannulation (blood sampling)
Exclusion criteria
Exclusion criteria: * Having a history of medical or surgical events that may significantly affect the study outcome * Medical drug use accept incidental use of paracetamol * (Chronic) disease which might influence the study outcomes e.g. diabetes mellitus or any other endocrine disorder, active cardiovascular disease, hepatic disease, renal disease, cancer , bowel disease * Milk protein or lactose intolerance or allergy * Alcohol consumption of >14 (women) or > 21 (men) units per week * Drug abuse * Moderate intense physical activity (exercise) for more than 5 hours/week * Reported weight loss or weight gain of > 3 kg in the month prior to pre-study screening * Reported slimming diet, or medically prescribed diet * Having a habitual diet with a protein content of 30 En% * Reported vegan or macrobiotic life-style
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The ratio of isotopic enrichment (15N/13C) of (total) amino acids of the test meal and the blood plasma as determined by (gas chromatography*) isotope ratio mass spectrometry ((GC)-IRMS). | — |
Secondary
| Measure | Time frame |
|---|---|
| Measurement of total N, urea and ammonia by kinetic UV assay and 15N and 13C enrichment by GC/IR-MS in urine will be assessed. | — |
Countries
Netherlands
Outcome results
None listed