Obstructive cholestasis
Conditions
Interventions
None listed
Sponsors
Medisch Universitair Ziekenhuis Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Patients undergoing pp Whipple Age >= 18 and
Exclusion criteria
Exclusion criteria: Jejunostomy Lactation, pregnancy and planning of pregnancy Inflammatory bowel disease Alcohol or drugs abuse within 1 year Inborn errors of bile salt synthesis
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main study parameter is the net organ flux of FGF19 across abdominal organs calculated by measuring FGF19 levels in human plasma using an enzyme-linked immuno-sorbent assay (ELISA) in cholestatic versus non-cholestatic patients and drained patients. Human plasma will be obtained during surgery from 7 vessels i) radial artery, ii) mesenteric superior vein, iii) mesenteric inferior vein, iv) renal vein, v) splenic vein, vi) hepatic vein and vii) portal vein to calculate net organ fluxes. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary parameters are expression of genes related to bile salt and FGF19 signaling in enterohepatic tissues (liver, jejunum, gallbladder, common bile duct and white adipose tissue), genes implicated in glucose and lipid homeostasis, FGF19 levels in bile, gut microbiota, cholestatic itch and bile salt composition in urine. | — |
Countries
Netherlands
Outcome results
None listed