function of the heart small for gestational age
Conditions
Interventions
None listed
Sponsors
Obstetrie & Gynaecologie
Eligibility
Inclusion criteria
Inclusion criteria: - Singleton pregnancies between 24 and 34 weeks gestation diagnosed with FGR (AC * 10th percentile/ decline in growth pattern * 40 percentiles) undergoing antenatal steroids (AS) (betamethasone 12 mg i.m. 2 doses 24 hours apart) for medical indication. - Singleton pregnancies between 24 and 34 weeks gestation with AGA fetuses (EFW * 10th percentile, AC* 10th percentile, normal growth pattern, normal umbilical artery (UA) and uterine arteries (AAUt) Dopplers) undergoing AS for medical indication.
Exclusion criteria
Exclusion criteria: Maternal age under 18 years Not capable of speaking and reading the Dutch language Multiple pregnancies Congenital abnormalities Abnormal fetal karyotype
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Detecting changes in the fetal hemodynamic system before and after admistration of antenatal corticosteroids in the growth restricted fetus (FGR) compared with the appropriate for gestational age (AGA) fetus. Data about Doppler parameters will be expressed as the number of standard deviations from the respective normal means for gestation (* value). The measurements will be analyzed for each fetus and compared before and after treatment; each fetus will be considered as its own control. The data obtained on the control day (baseline examination) will be considered for significant difference with the measurements acquired in the subsequent ultrasounds. | — |
Secondary
| Measure | Time frame |
|---|---|
| Changes in the methylation of the HSD11B2 gene after administration of corticosteroids in the growth restricted fetus (FGR) compared with the appropriate for gestational age (AGA) fetus. Methylation analysis will be performed by pyrosequencing. These results are calculated by a computer program and shown as a percentage. Immune parameters will be analyzed by multicolor flowcytometry, rtPCR analysis of RNA, and immunohistologic stainings. | — |
Countries
Netherlands
Outcome results
None listed