Co-stimulatory and -inhibitory molecules on tumor-infiltrating lymphocytes from cholangiocarcinoma

Co-stimulatory and -inhibitory molecules on tumor-infiltrating lymphocytes from cholangiocarcinoma - Co-stimulatory and -inhibitory molecules in CCA

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON43309

Enrollment

102

Registered

2016-08-15

Start date

2016-09-01

Completion date

Unknown

Last updated

2026-06-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

bile duct cancer cholangiocarcinoma

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Adult cholangiocarcinoma (CCA) patients that will undergo surgery for this disease.

Exclusion criteria

Exclusion criteria: Patients who refuse blood donation/participation in the study Patients with a severe immunocompromised condition, or patients taking immunosuppressive medication

Design outcomes

Primary

Measure	Time frame
The main study parameters are: 1) Frequencies and absolute cell counts of different lymphocyte populations that together compose the tumor-infiltrating lymphocyte pool. 2) Expression of co-stimulatory/co-inhibitory molecules on TIL vs lymphocytes isolated from tumor-free liver tissue vs circulating lymphocytes 3) Effect of blocking co-inhibitory molecules or stimulating co-stimulatory molecules expressed by TIL on their function (proliferation and cytokine production) in ex vivo co-culture experiments	—

Measure

Time frame

The main study parameters are: 1) Frequencies and absolute cell counts of different lymphocyte populations that together compose the tumor-infiltrating lymphocyte pool. 2) Expression of co-stimulatory/co-inhibitory molecules on TIL vs lymphocytes isolated from tumor-free liver tissue vs circulating lymphocytes 3) Effect of blocking co-inhibitory molecules or stimulating co-stimulatory molecules expressed by TIL on their function (proliferation and cytokine production) in ex vivo co-culture experiments

—

Secondary

Measure	Time frame
Depending on the co-inhibitory molecules detected on TIL, immunohistochemistry will be performed on residual formalin-fixed paraffin-embedded tumor tissue that is regularly stored to identify the expression of the ligands of these co-inhibitory molecules (for instance PDL1+2 and GAL9) on tumor (infiltrating) cells.	—

Countries

Netherlands

Outcome results