Costimulatory and -inhibitory molecules on tumor-infiltrating lymphocytes from colorectal carcinoma and its metastases

Costimulatory and -inhibitory molecules on tumor-infiltrating lymphocytes from colorectal carcinoma and its metastases - Costimulatory and -inhibitory molecules in CRC

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON43140

Enrollment

677

Registered

2017-01-16

Start date

2017-04-04

Completion date

Unknown

Last updated

2024-02-28

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

bowel cancer colorectal carcinoma

Interventions

co-inhibitory molecules

colorectal carcinoma

costimulatory molecules

T cell

Eligibility

Age

18 Years to 64 Years

Inclusion criteria

Inclusion criteria: Adult colorectal carcinoma (CRC) patients that will undergo surgery for CRC or its metastases

Exclusion criteria

Exclusion criteria: Patients who refuse blood donation/participation in the study Patients with a severe immunocompromised condition, or patients taking immunosuppressive medication

Design outcomes

Primary

Measure	Time frame
The main study parameters are: 1) Frequencies and absolute numbers of different lymphocyte populations that together compose the tumor-infiltrating lymphocyte pool 2) Expression of costimulatory and co-inhibitory molecules on TIL versus lymphocytes isolated from tumor-free tissue versus circulating lymphocytes 3) Effect of blocking co-inhibitory molecules or stimulating costimulatory molecules expressed by TIL on their function (proliferation and cytokine production) in ex vivo culture experiments	—

Measure

Time frame

The main study parameters are: 1) Frequencies and absolute numbers of different lymphocyte populations that together compose the tumor-infiltrating lymphocyte pool 2) Expression of costimulatory and co-inhibitory molecules on TIL versus lymphocytes isolated from tumor-free tissue versus circulating lymphocytes 3) Effect of blocking co-inhibitory molecules or stimulating costimulatory molecules expressed by TIL on their function (proliferation and cytokine production) in ex vivo culture experiments

—

Secondary

Measure	Time frame
Depending on the co-inhibitory molecules detected on TIL, immunohistochemistry will be performed on residual formalin-fixed paraffin-embedded tumor tissue that is regularly stored to identify the expression of the ligands of these co-inhibitory molecules (e.g. PDL-1+2, GAL-9) on tumor (infiltrating) cells. If possible, lymph node (when part of excision preparation) will be analyzed.	—

Countries

The Netherlands

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)