adipose tissue chronic low-grade inflammation inflammation of the adipose tissue
Conditions
Interventions
None listed
Sponsors
Radboud Universitair Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: group 1 (males and females): age: 35-75 years, body mass index (BMI) of 19-25 kg/m2;group 2 (males and females): age: 35-75 years, body mass index (BMI) of >25 kg/m2 ;group 3 (males and females): age: 35-75 years, body mass index (BMI) of >25 kg/m2, diagnosis of T2DM
Exclusion criteria
Exclusion criteria: any presence of a chronic or acute inflammation and/or autoimmune disorder, any anti-inflammatory medication, metabolic diseases, endocrine diseases and chronic and/or acute inflammatory diseases (high sensitivity C-reactive protein > 1 mg/liter)
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To determine metabolic pathways important for inflammatory function of CD14+ cells from blood, visceral and subcutaneous adipose tissue by gene expression analysis (human microarray platform by affymetrix) using a bioinformatics approach. To determine the intracellular metabolism of CD14+ cells from blood and adipose tissue by measurements of metabolites in cell lysates or supernatants of the (cultured) immune cells and by analysis of intracellular metabolic signalling pathways on protein and gene expression level. | — |
Secondary
| Measure | Time frame |
|---|---|
| To determine the differences in inflammatory potential of CD14+ cells isolated from lean, obese and diabetic subjects by histological assessment of adipocyte size, macrophage infiltration and formation of crown-like structures in the different adipose tissue depots and by measurements of plasma cytokines and cytokines released upon stimulation of cultured cells isolated from the blood. | — |
Countries
Netherlands
Outcome results
None listed